Studies on the relation between heme and nucleic acid syntheses in erythroid cell. II. Nucleic acid synthesis in erythroblast of anemic rat treated with aminopterin and bromouracil.

With the bone marrow of anemic rats, which had received the repeated injections of phenylhydrazine once a day for three to four days, the effects of aminopterin and bromouracil on the nucleic acid metabolism of erythroblasts were observed in vivo experiment. The injection of aminopterin suppreed DNA synthesis with the lowered labeling index as observed by the incorporation of Hthymidine into DNA in vitro. But the grain count per cell showed the level similar to that of anemic control. RNA synthesis was not interfered by AP injections. These results indicate that AP mainly supprees the thymidilate kinase. Bromouracil showed no such effect even on the administration of a large dose. On the basis of the data obtained from the experiment by using AP, a discuion was made on the correlation between DNA synthesis, nuclear function and the cell specialization. ∗PMID: 4240919 [PubMed indexed for MEDLINE] Copyright c ©OKAYAMA UNIVERSITY MEDICAL SCHOOL Acta Merl. Okayama 22, 319-329 (1968) STUDIES ON THE RELATION BETWEEN HEME AND NUCLEIC ACID SYNTHESES IN ERYTHROID CELL II. NUCLEIC ACID SYNTHESIS IN ERYTH:ROBLAST OF ANEMIC RAT TREATED WITH AMINO. PTERIN AND BROMOURACIL